yes, on a dare once, at an open mike night. i did fine but did not pursue it because the whole housewife-turned-comedienne thing has been done to death and i DO NOT want to be compared in any way to roseanne barr...

it helped me INCREDIBLY that the guy who went before me totally sucked...talked all about his dog shredding his dirty underpants in the middle of the floor, in a manner that was not funny but uncomfortable and thoroughly disgusting and people barely clapped when he left. i started by introducing myself and saying, "let me just put you all at ease and establish RIGHT NOW that i will NOT talk to you about dogs, underpants, any of that...in fact, i'm not even WEARING underpants." they clapped and hooted and hollered and a guy yelled "show us!!!!" and i just turned to him and said "HEY!" gave him my "stern mommy look" while pointing my finger at him. pause, continue, explaining that you've just seen my "strn mommy look"...so the whole "stern mommy look" thing became both a good tangent and a running joke. my best advice is, don't be intimidated by hecklers...USE them, and have ideas about what you want to say. (and if you decide in the end like i did that you have a face for radio, WRITE instead.)
durg, brutus...good for you, very brave. find out when your local comedy club has open mike night, and become a regular. you'll get good practice, you'll become recognizable, and who knows who will see you there! good luck and tell us how it goes!!!